Program

Tuesday April 8^st 2014

9h00-9h15 :  Welcome address (S. Cianférani)

9h15-10h : Opening lecture - Albert Heck (Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Research, Utrecht University, The Netherlands)

 From the top down; mass spectrometry and its role in studying intact proteins and protein complex structure, dynamics, and assembly

10h00-11h00: session 1 : Protein ionization – Chairman E. De Pauw

10h-10h40 : Transferring Proteins from Solution into the Gas Phase:How Well Do We Understand the ESI Process?
Lars Konermann (Department of Chemistry, The University of Western Ontario, London, Canada)

10h40-11h00 : Surface Acoustic Wave Nebulization allows desorption of MDa species from a flat surface

Ann-Kathrin Stark (Laboratoire de Biologie à Grande Echelle, CEA Grenoble, France)

11h00-11h30 : coffee break /posters

11h30-12h50: session 1 : Gas phase protein conformations – Chairman

11h30-12h10: Ion Mobility:  Cross Sections and Solution Structures       
Michael T. Bowers (Department of Chemistry and Biochemistry, University of Santa Barbara, USA)

12h10-12h30 : Conformational distribution of bradykinin 3+ revealed by cold-ion spectroscopy and differential ion mobility spectrometry
Liudmila Voronina (Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland)

12h30- 12h50 : Action FRET: Probing the molecular conformation of mass-selected gas phase ions with FRET detected by mass-spectrometry only

Steven Daly (CNRS and University of Lyon 1, UMR5306, Institut Lumière Matière University of Lyon, France)

13h00-14h30 : Lunch

14h30-16h10: session 2 : Recombinant protein characterization by native MS – Chairman J. Benesch

 14h30-15h10: Probing Protein Stability, Unfolding and Dissociation with Variable Temperature Mass Spectrometry and Variable Temperature Ion Mobility Mass
Perdita Barran (Manchester Institute of Biotechnology, University of Manchester, Manchester, UK)

15h10-15h30: Lipid A transport by the asymmetric heterodimeric ABC transporter TmrAB revealed by native mass spectrometry

 Chérine Béchara (Department of Chemistry, University of Oxford,UK)

15h30-15h50: Real-time Monitoring of Protein Phosphorylation Using High-Resolution Native MS
Philip Lössl (Biomolecular Mass Spectrometry and Proteomics. Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, The Netherlands)

15h50-16h10:  Novel sheathless CE-MS interface as an original and powerful infusion platform for nanoESI study: from intact proteins to high molecular mass noncovalent complexes
Rabah Gahoual (Laboratoire de Spectrométrie de Masse des Interactions et des Systèmes -LSMIS, CNRS-UMR 7140, University of Strasbourg, France )

16h10-17h00: coffee/posters

17h00- 18h15: Flash communications from instrument manufacturers – Chairman

17h00-17h10 : Structural analysis of membrane proteins and antibodies by uniform low field Ion Mobility Mass Spectrometry (Joachim Thiemann, Agilent Technologies Inc., Waldbronn, Germany)

17h10-17h20: How Waters is leading innovation for the native mass spectrometry (Laetitia Denbigh, Waters corporation, Manchester, UK)

17h20-17h30: Analysis of intact macromolecular assemblies under native conditions on a bench top Orbitrap MS system (Yue Xuan, Thermo Fisher Scientific, Bremen, Germany)

17h30-17h40: Improving high-mass performance of conventional Time-of-Flight platforms for native MS by activation and collisional cooling in the interface region (Jan Commandeur, MS Vision, Almere, The Netherlands)

17h40-17h50: Analysis of Ions Generated From Native Spray Conditions with Trapped Ion Mobility Spectrometry (TIMS) (Mark Ridgeway, Bruker Daltonics Inc., Billerica, USA)

17h50-18h15: Q&A (Discussion)

18h30 – Bus to city-center

19h30: Social Diner at “Brasserie Flo” (8 Rue Outre, 67000 Strasbourg)

Wednesday April 9^nd 2014

9h-10h20: session3 : Structural Biology (Part I) – Chairman K. Thalassinos

9h00-9h40: Ion Mobility-Mass Spectrometry of Complex Carbohydrates
Kevin Pagel (Fritz Haber Institute of the MPG and Free University Berlin, Berlin, Germany)

 9h40-10h00: Native mass spectrometry and ion mobility of protein-nucleic acid interactions: shape, stability and stoichiometry  
Esther M. Martin (Biomolecular & Analytical Mass Spectrometry Group, University of Antwerp, Belgium)

10h00-10h20 : Ion mobility-collision induced dissociation-mass spectrometry for the structural analysis of polydisperse small heat shock protein ensembles
Dale A. Shepherd (Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, UK)

10h20-11h00 : coffee/posters

11h00-12h30 : session 4 : Structural Biology (Part II) – Chairman A. Aschcroft

11h00-11h40: Structural Biology in the Gas Phase: New Approaches for Conformationally-selective Inhibitor Screening and Multiprotein Topology Mapping
Brandon Ruotolo (Department of Chemistry, University of Michigan, Ann Arbor, USA)

11h40-12h00: Application of IM-MS and HDX MS for mapping conformational changes occurring upon allosteric inhibition of enzymes: The ATP-phosporibosyltransferase case
Kamila J. Pacholarz (School of Chemistry, University of Edinburgh, Edinburgh, UK)

12h00-12h20 : Combined structural study of lasso peptides: Hydrogen/Deuterium exchange coupled with ion mobility spectrometry
Emeline Hanozin (Mass spectrometry Laboratory, University of Liege, Belgium)

12h30-14h00 : Lunch

14h00-15h40 : session 5 : Native MS and IM-MS for nucleaic acids and protein/ligand systems – Chairman F. Sobott

14h00-14h40: Nucleic Acids in the Gas Phase    
Valérie Gabélica (University of Bordeaux, IECB, Inserm U869, ARNA Laboratory, Bordeaux, France)

14h40-15h00: A step towards high throughput drug screening using ion mobility mass spectrometry unfolding experiments    
Ganesh N. Sivalingam (University College London, London, UK)

15h00-15h20 : Real-Time conformational characterization of protein-ligand complexes by Native MS coupled to Ion Mobility
Johann Stojko (Laboratoire de Spectrométrie de Masse BioOrganique - LSMBO), IPHC-DSA, University of Strasbourg, CNRS UMR7178, France)

15h20-15h40 : Mass spectrometry defines the C-terminal dimerization domain and enables modeling of the structure of full-length OmpA
Julien Marcoux (Department of Chemistry,  University of Oxford, UK)

15h40-16h20 : Closing  lecture
Alain Van Dorsselaer (University of Strasbourg, France)

16h20-16h30 : closing remarks (S. Cianférani)

16h45 : bus to train station